讲述刑事警察局的法庭犯法调查员如何在作案现场取得证据破案的故事。就像本剧的姐妹剧《犯罪现场调查：迈阿密篇第七季》一样。调查员的名言是“逝世尸会说话”，他们利用指纹、鞋印、子弹壳、血迹、毛发、纤维、尸体伤痕等渺小证据，经过仔细的分析研究后，寻得破案的要害。
CSI: New York, the third incarnation of the CSI: Crime Scene Investigation franchise, is a crime drama about forensic investigators who use high-tech science to follow the evidence and solve crimes in The Big Apple. Detective Mack "Mac" Taylor is a dedicated and driven crime scene investigator who believes that everything is connected and for everyone there is a story. He and his partner, Detective Stella Bonasera, a workaholic and a jack-of-all-trades, share a passion for the job. They lead a team of experts amid the gritty and kinetic city that never sleeps. Their team includes Danny Messer, a Brooklyn-born investigator with rugged good looks, an unflappable spirit and a colorful family history, and Dr. Sheldon Hawkes, a reclusive coroner who walked away from a promising surgical career after the traumatic loss of two patients. Joining them is homicide Detective Don Flack, an edgy, hardcore investigator with a quick wit, impressive forensic insight and limited patience with potential suspects. Rounding out the team is Aiden Burn, a smart and sexy investigator whose chameleon-like behavior allows her to adapt to any situation at any time. These skilled investigators, who see New York City in a whole different light, follow the evidence as they piece together clues and eliminate doubt to ultimately crack their cases.
