故事背景是设定在今日美国佛罗里达州的迈阿密。在北美平均每一集约有2千万个观众收看，使得它成为星期一收视第一的电视系列剧，也是最多人收看的电视系列剧。这个系列电视系列剧在全世界几十个国家都有播出，它是由亚特兰提斯同盟（Alliance Atlantis）行销至国际间。
小组成员调查神秘与不寻常的死因，以判定是谁以及如何杀了他们。成员们也会解决一些其他的重罪，例如强奸罪的采证，但这个系列电视系列剧的主题仍以谋杀案件为主。
本剧集场景发生在佛罗里达州的迈阿密，以当地办案人员为故事主轴，描写他们是如何地透过高科技的辅助，以及巨细靡遗的传统办案手法，破解一个又一个待解的刑案。隶属于迈阿密犯罪鉴证科的犯罪现场调查小组，频频出入各种案发现场寻找证据和蛛丝马迹，小组成员利用最先进的科技手段， 把表面上没有任何意义的线索拼凑成不可辩驳的证据，抽丝剥茧重组案情，如《犯罪现场调查第三季》剧情那样让犯罪分子无处可逃……
CSI: Crime Scene Investigation took television by storm in 2000 with its innovative and cutting-edge updating of a standard series concept – in this case, the cop show. Suddenly, instead of simply whodunit, solving crimes was a matter of howdunit, and with what size shoe, and whether anyone left behind traces of evidence that the blue ALS light could pick up. Now that gritty approach to crime known as forensic science makes its way from Las Vegas to the streets of Florida in CSI: Miami. A whole new team of crime scene investigators is on hand to probe the seamy underside of what some refer to as Cuba North and others call Georgia South. Consider it a tropical blend of people, culture, nightlife and, of course, the occasional killing or two. Just as you do in Vegas, come for the crime, stick around for the clues. And there"s always a clue.
